Correlative Light and Electron Microscopy Reveals the HAS3-Induced Dorsal Plasma Membrane Ruffles

Figure 1

Utilization of gridded coverslips for localization of cultured cells for correlative imaging. Low magnification images showing an overview of transiently transfected cells imaged by CLSM and DIC (a) and the corresponding area imaged by SEM (b). Arrows in (a) indicate cells that were detached in the subsequent preparation steps for scanning electron microscopy. Scale bar 100 μm.

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(b)