Correlative Light and Electron Microscopy Reveals the HAS3-Induced Dorsal Plasma Membrane Ruffles

Figure 3

GFP-HAS3 expression induces plasma membrane ruffling. Confocal 3D projection of transiently transfected MCF-7 cells imaged by CLSM (a) and the corresponding area by SEM (b). Arrows in (b) indicate GFP-HAS3-positive cells with plasma membrane ruffling, while negative cells (asterisks) have smoother dorsal surface. Arrowheads in (a) and (b) indicate a cell that was lost during SEM processing. Stable, inducible transfections were utilized to quantify the dorsal ruffling of MCF-7 cells, which was significantly increased upon induction of GFP-HAS3. Significant difference () in quantified parameters of ruffling is indicated by an asterisk () in table (c). in both groups. A GFP-HAS3-positive cell treated with hyaluronidase before fixation is shown with CLSM and SEM in (d) and (e), respectively. Scale bars 10 μm in (a) and 5 μm in (d).

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