Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay

Figure 3

Western blot analysis of HEK293T cells treated with 0–200 ng/mL Wnt3a for 24, 48, or 72 h. The membrane was incubated with cyclin D1 and c-Myc specific antibodies. β-actin antibody was used as loading control. For quantification, the protein levels were normalized to the level of β-actin and the untreated control was set to 100%.