Measured Effects of Wnt3a on Proliferation of HEK293T Cells Depend on the Applied Assay

Figure 5

Mitochondrial activity of Wnt3a treated HEK293T cells. (a) Exponentially growing HEK293T cells were exposed to different Wnt3a concentrations for the indicated times. Protein extracts were prepared and used for western blot analysis. The membrane fractions were incubated with antibody against SDHA or, as loading control, against β-actin. (b and c) HEK293T cells were subjected to immunocytochemical analysis. SDHA protein was detected by green fluorescence via fluorescence microscopy at 200x magnification. Bars are showing band intensity expressed as percentage in comparison to the untreated control (vertical axis), which was normalized to 100%.

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