Selective Activation of Cancer Stem Cells by Size-Specific Hyaluronan in Head and Neck Cancer

Figure 6

Analyses of 200 kDa-HA-mediated survival protein (cIAP1) expression in cells. (a) Detection of the expression of cIAP1 by anti-cIAP1-mediated immunoblotting using cell lysate isolated from cells treated with different sizes of HA [e.g., no HA ((A), lane 1), 5 kDa-HA ((A), lane 2), 20 kDa-HA ((A), lane 3), and 200 kDa-HA ((A), lane 4)] for 3 days. (b) Detection of the expression of cIAP1 by anti-cIAP1-mediated immunoblotting using cell lysate isolated from cells transfected with negative miRNA control [treated with no HA ((A), lane 1) or with 200 kDa-HA for 24 h ((A), lane 2)] or treated with anti-miR-10b inhibitor with no HA ((A), lane 3) or with 200 kDa HA addition for 3 days ((A), lane 4). The amount of actin detected by anti-actin-mediated immunoblot ((a): (B), lane 1–4; (b): (B), lane 1–4) in each gel lane was used as a loading control.

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