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(b)
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Figure 2: Labeling pattern of fixed hemocytes isolated from Litopenaeus vannamei after incubation with FITC-conjugated lectins. Many hemocytes express ligands differentially: (a) wheat germ agglutinin (WGA), (b) peanut agglutinin (PNA), and (c) Lycopersicon esculentum agglutinin (LEA). Hemocytes were evaluated by interference contrast microscopy and epifluorescence microscopy. Scale bar = 5 μm. (d) Percentage of total hemocytes and cell types of positive cells labeled by FITC-conjugated lectins. For each treatment, digital images were used to analyze at least 500 hemocytes. The percentage of positive (i.e., fluorescing) cells per sample was calculated, as defined with reference to the total hemocyte number. Data represent mean ± SD.