(a)
(b)
(c)
(d)
Figure 3: Enzyme cytochemistry of adhered hemocytes isolated from Litopenaeus vannamei. The first column of micrographs corresponds to the control cells incubated without the enzyme substrate and the second column shows enzymatic activities. (a) Acid phosphatase, (b) nonspecific esterase (alpha-naphthyl esterase), and (c) specific esterase (AS-D chloroacetate esterase). Scale bar = 5 μm. (d) Percentage of adhered hemocytes of L. vannamei, which react with the substrates for different enzymes, along with cell types of positive cells. Data represent mean ± SD. Letters indicate significant differences () in percentage of positive cells.