Table 1: Lectins used to investigate cell surface oligosaccharides domains in hemocytes and their competitive sugars used as controls for lectin-binding.

LectinLatin nameSourceResuspension solutionSpecificity sugarInhibition

Con-ACanavalia ensiformis
(Sigma, C7642)
Jack beanNaCl 0.9% pH 6.5 with 5 mM CaCl2a-D-Mannose, a-D-glucoseGlucose
WGATriticum vulgaris
(Sigma, L4895)
Wheat germPBS pH 7.4(D-GlcNAc), NeuNAcN-Acetylglucosamine
PNAArachis hypogaea
(Sigma, L7381)
PeanutPBS pH 7.4-Galactose(1-3) galNAcGalactosamine
UEA-IUlex europaeus
(Sigma, L9006)
Gorse, furzeNaCl 0.9%a-L-FucoseFucose
LEALycopersicon esculentum
(Sigma, L0401)
Tomato(glcNAc)3N-Acetylglucosamine

GalNAc = N-acetyl-galactosamine, GlcNAc = N-acetyl-glucosamine, and NeuNAc = N-acetylneuraminic acid.
Competing sugar used in the study at 0.2 M.
10 mM HEPES, 0.15 M NaCl, 0.1 mM Ca2+, 0.08% sodium azide, and 5 mg mL−1   β-cyclodextrin.