The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells

Figure 5

The mesenchymal stem cells (MSCs) characterization by flow cytometry, differentiation, and CFU-F assay. (a) Expression of surface antigens in hAD-MSCs, and transduced hAD-MSCs as determined using flow cytometry. (b) Control: the second passage of hAD- MSCs and transduced hAD-MSCs. Osteogenic: staining of hAD-MSCs and transduced hAD-MSCs by Alizarin Red S reagent; calcium deposits stained bright orange-red around hAD-MSCs differentiated into osteocytes on the 21th day. Adipogenic: staining of hAD-MSCs and transduced hAD-MSCs after 2nd passage by Oil Red O reagent; intracellular vesicles of oil accumulated and stained bright red within hAD-MSCs differentiated into adipocytes on the 21th day. (c) Clonogenic capacity was measured by colony forming unit-fibroblast (CFU-F) assay. The results are represented as the means ± SD.

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