Engineering of L-Plastin Peptide-Loaded Biodegradable Nanoparticles for Sustained Delivery and Suppression of Osteoclast Function In Vitro

Figure 4

Immunoblotting (IB) analysis of the time-dependent effect of NP1 and NP3 on the phosphorylation of endogenous LPL. The equal amount of lysates made from osteoclasts treated with NP1 (lanes 1 and 3) and NP3 (lanes 3 and 4) for 4h (lanes 1 and 2) and 6h (lanes 3 and 4) was used for immunoprecipitation (IP) with an LPL antibody. IP with a nonimmune serum is shown in lane 5. Immunoprecipitates were subjected to IB with an antibody to p-Serine (top panel). Blot was stripped and reprobed with an antibody to LPL (bottom panel). Percent inhibition of phosphorylation of the representative experiment with NP1 is 78% at 4h and 53% at 6h as compared with corresponding NP3 control in lanes 2 and 4. These results represent one of the two experiments performed with similar results.