International Journal of Cell Biology / 2019 / Article / Fig 2

Research Article

Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis

Figure 2

Both TGF-β neutralization and rhIL-15 treatment inhibit the “spontaneous EMT”. (a) Immunofluorescent staining of E-cadherin (epithelial marker) and vimentin (mesenchymal marker) expressions at day 5 in RPTEC cells under standard (complete REBM) and “spontaneous EMT” conditions, in presence or absence of neutralizing TGF-β1 antibody (5 μg/mL) and/or rhIL-15 treatment (1 ng/mL). (b) E-cadherin (epithelial marker) and N-cadherin (mesenchymal marker) expressions were analyzed by Western blot at day 5 in RPTEC cells, under the same culture cell conditions and treatments. Bar charts represents E-cadherin and N-cadherin expression normalized to tubulin (n=3).

(a)
(b)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.