Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis
IL-15 inhibited TGF-β-induced Snail1 expression in HK-2 cells through phospho-C-Jun upregulation. (a) Western blot analysis of Snail1 expression after a 48h-rhTGF-β1 stimulation (3 ng/mL) in presence or absence of rhIL-15 treatment (1 ng/mL). Bar chart represents Snail1 expression normalized to β-actin (p<0.05, n=6, ±SEMs). (b) Western blot analysis and (c) Immunofluorescent staining of phospho-c-Jun expressed after 1h to 48h of rhIL-15 treatment (1 ng/mL) ± the specific JNK inhibitor SP600125. Bar chart represents phospho-c-Jun expression normalized to GAPDH (p<0.01, p<0.05, n=3, ±SEMs). To visualize cells, cell nuclei were stained with DAPI (Lower panels). Immunofluorescence data are representative of three independent experiments.
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