Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro

Figure 4

Selective AVICs marker gene expression analyses after osteogenic (OS) media induction in avian adult AVICs in vitro. (a) (I–VII): qRT-PCR data show that Col1a1, Vimentin, and Adamts9 have been downregulated by 0.97 fold, 0.83 fold, and 0.62 fold, respectively (I, IV, and VII). In addition, Sm22α, Fibronectin, N-cadherin, Adamts5, and PiT2 have been upregulated by 17.25 fold, 8.51 fold, 4.42 fold, 0.23 fold, and 3.36 fold, respectively (II, III, V, VI, and VIII). (b) (I–III): similarly, positive regulators of Wnt signaling, β-catenin and Wnt3a, have been upregulated by 1.44 fold and 4.73 fold, respectively (I and II). In contrast, the negative modulator of Wnt signaling, Dkk1 mRNA, is decreased by 0.27 fold (III). Statistical significance was determined by Student’s -test, where denotes , denotes and denotes and .

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(b)