Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation In Vitro

Table 2

qRT-PCR results of comparison between uninduced control and OS-induced AVICs.

			OS-induced AVICs
Osteogenic marker genes	Osteopontin, Runx2, ALP, Msx2, Osterix		All are upregulated
Negative regulatory molecule of bone cell differentiation	Asporin		Downregulated
AVICs marker genes	Fibroblast	Col1a1	Downregulated
	Fibroblast	Vimentin	Downregulated
	Myofibroblast	Sm22α	Upregulated
	Extracellular matrix molecule	Fibronectin	Upregulated
	Cell adhesion molecule	N-Cadherin	Upregulated
	Matrix metalloproteinase	Adamts5	Upregulated
	Matrix metalloproteinase	Adamts9	Downregulated
Intracellular phosphate level regulatory gene	Na-dependent Pi transporter	PiT2	Upregulated
Signaling-specific markers genes	Wnt signaling	β-Catenin	Upregulated
		Wnt3a	Upregulated
		Dkk1	Downregulated
	Other signaling-specific marker genes	Bmp2	Upregulated
		Smad 1	Downregulated
		Smad 5	Upregulated
		Smad 8	Upregulated
		Notch 1	Upregulated
		Sox 9	Downregulated