A New Rapid and Sensitive Spectrophotometric Method for Determination of a Biopolymer Chitosan

Table 1

Stability of color complex by the proposed method.

Time after color development (min)	Absorbance (555 nm) with the following amount of chitosan (μg/mL)
	5	10	25
5	0.086	0.151	0.410
10	0.086	0.152	0.412
15	0.091	0.153	0.415
20	0.095	0.154	0.411
25	0.098	0.152	0.410
30	0.098	0.152	0.411
35	0.101	0.154	0.412
40	0.100	0.152	0.411
45	0.100	0.154	0.409
50	0.102	0.157	0.409
55	0.097	0.155	0.404
60	0.100	0.157	0.405
90	0.092	0.153	0.402
120	0.092	0.153	0.409
150	0.092	0.150	0.407
180	0.092	0.150	0.414
1080 (18 h)	0.046	0.080	0.231

The values are means of four replicates.