Factors Involved in the In Vitro Fermentability of Short Carbohydrates in Static Faecal Batch Cultures

Figure 2

Dionex-HPLC chromatogram of the galactooligosaccharide mixture GOS³ breakdown over 24 h measured in cell-free fermentation supernatants. Static faecal batch culture fermentations were carried out in an anaerobic cabinet with a starting concentration of GOS³ of 0.5% (w/v). Samples were taken after 2, 4, 6, 8, and 24 h of incubation. The oligosaccharide which is represented by peak 22 seemed to be resistant to fermentation.