International Journal of Carbohydrate Chemistry / 2012 / Article / Tab 1

Research Article

Partial Chemical Characterization of Immunomodulatory Polysaccharides from Plantago palmata Hook. f. s. Leaves

Table 1

Molecular weights of polymers obtained from Plantago palmata leaves by aqueous extraction (50°C, 3 hours), concentration and extensive dialysis (PS50) and further filtration (PS50F) on a Millipore cellulose filter (0.22  m). SEC analysis referring to a calibration curve obtained with pullulan standards (n = 3).

PolymerElution volume Polydispersity
(mL)(g/mol)(g/mol)(g/mol)(g/mol)( / )

11.8 to 13.8 mLa1129867 ( 39000)1192500 ( 51000)1255767 ( 65000)1190167 ( 37000)1.05
PS5013.8 to 16.8 mLb222537 ( 16000)271913 ( 12000)322567 ( 8000)303167 ( 12000)1.22
16.8 to 19.6 mLc39485 ( 650)11818 ( 720)14856 ( 760)9895 ( 550)1.24

11.8 to 13.8 mL1189650 ( 9000)1278550 ( 14000)1369650 ( 20000)1262000 ( 13000)1.07
PS50F13.8 to 16.8 mL230515 ( 1000)277390 ( 620)325485 ( 605)304600 ( 6000)1.20
16.8 to 19.6 mL10338 ( 250)12860 ( 720)16068 ( 960)10597 ( 3300)1.24

 = Number average molecular weight;  = Weight average molecular weight;  = Z average molecular weight;  = Peak molecular weight.
aCorresponds to fraction F2-1 (Figure 1).
bCorresponds to fraction F2-2 plus part of F2-3 enclosing the tail of the major peak.
cComprehends the peak skimmed from the major peak (corresponds to the end of fraction F2-3 plus part of F3-1).

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