Table of Contents
International Journal of Carbohydrate Chemistry
Volume 2013, Article ID 539636, 10 pages
Research Article

A Green Approach to Synthesize Silver Nanoparticles in Starch-co-Poly(acrylamide) Hydrogels by Tridax procumbens Leaf Extract and Their Antibacterial Activity

1Department of Chemistry, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh 515 003, India
2Department of Polymer Science and Engineering, Pusan National University, Busan 609-735, Republic of Korea
3Department of Microbiology, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh 515 003, India
4Department of Polymer Science & Tech, Sri Krishnadevaraya University, Anantapur, Andhra Pradesh 515 003, India

Received 31 August 2013; Accepted 13 November 2013

Academic Editor: Roland J. Pieters

Copyright © 2013 Siraj Shaik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A series of starch-co-poly(acrylamide) (starch-co-PAAm) hydrogels were synthesized by employing free radical redox polymerization. A novel green approach, Tridax procumbens (TD) leaf extract, was used for reduction of silver ions (Ag+) into silver nanoparticles in the starch-co-PAAm hydrogel network. The formation of silver nanoparticles was confirmed by UV-visible spectroscopy (UV-Vis), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (X-RD) studies. 22% of weight loss difference between hydrogel and silver nanocomposite hydrogel (SNCH) clearly indicates the formation of silver nanoparticles by TGA. TEM images indicate the successful incorporation of silver nanoparticles ranging from 5 to 10 nm in size and spherical in shape with a narrow size distribution. These developed SNCHs were used to study the antibacterial activity by inhibition zone method against gram-positive and gram-negative bacteria such as Bacillus and Escherichia coli. The results indicated that these SNCHs can be used potentially for biomedical applications.