Table of Contents
International Journal of Carbohydrate Chemistry
Volume 2013 (2013), Article ID 846565, 7 pages
http://dx.doi.org/10.1155/2013/846565
Research Article

Analgesic Activity of a Glucan Polysaccharide Isolated from Agaricus blazei Murill

1Department of Organic and Inorganic Chemistry, Federal University of Ceará, P.O. Box 12200, 60455-760 Fortaleza, CE, Brazil
2Department of Biochemistry and Molecular Biology, Federal University of Ceará, P.O. Box 12200, 60455-760 Fortaleza, CE, Brazil

Received 9 July 2013; Revised 30 July 2013; Accepted 31 July 2013

Academic Editor: R. J. Linhardt

Copyright © 2013 Maria Leônia C. Gonzaga et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A water soluble polysaccharide and its selective precipitated fractions (F1, F2, and F3) were isolated from hot water extract of fruiting bodies of Agaricus blazei Murril known as Agaricus brasiliensis to evaluate the analgesic activity of the materials in Swiss mice. The isolated material and its fractions were characterized by two-dimensional COSY and HMQC spectra as β-(1→6)-α-(1→4)-glucan-bound-protein polysaccharide constituted mainly by β-(1→6) glucan. The glucan-protein polysaccharide and its fractions were analyzed in relation to the protein, carbohydrate, and mineral elements contents. The analysis of the glucan-protein complex revealed a total carbohydrate content of 36.95% and protein conjugated content of 17.24%. The mineral elements found in larger proportion were K, Na, Ca, P, and Zn. The analgesic activity bioassay of the β-(1→6)-α-(1→4)-glucan bound-protein polysaccharide and its fractions in Swiss mice indicated a significant effect.