International Journal of Carbohydrate Chemistry

Research Article

Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin

Table 2

Disaccharide compositions of unfractionated and fractionated chondroitin sulfate samples from whale and bovine cartilage.


Fraction	ΔDi-0S	ΔDi-4S	ΔDi-6S	ΔDi-diS_E	ΔDi-diS_B	ΔDi-diS_D	ΔDi-triS

Whale cartilage

CS (untreated)	1.2	76.8	20.9	0.3	N.D.	0.7	Trace
A	1.2	75.7	22.8	0.1	N.D.	0.2	Trace
B	1.3	75.4	22.7	0.2	N.D.	0.4	Trace
C	1.5	75.7	22.4	0.1	N.D.	0.3	Trace
D	1.5	76.3	21.9	0.1	N.D.	0.2	Trace
E	1.5	76.7	21.0	0.2	N.D.	0.5	Trace
F	3.4	76.0	20.4	0.1	N.D.	0.1	Trace

Bovine tracheal cartilage

CS (untreated)	8.1	56.5	33.8	0.6	N.D.	1.0	Trace
A	6.7	61.3	30.9	0.5	N.D.	0.7	Trace
B	7.5	62.3	29.0	0.5	N.D.	0.7	Trace
C	7.3	61.7	29.7	0.5	N.D.	0.8	Trace
D	6.6	59.1	33.1	0.4	N.D.	0.7	Trace
E	6.4	55.7	36.9	0.3	N.D.	0.6	Trace
F	6.7	52.1	40.3	0.3	N.D.	0.6	Trace
G	5.4	50.0	43.4	0.4	N.D.	0.8	Trace
H	8.6	50.1	40.2	0.3	N.D.	0.9	Trace
I	8.2	48.7	41.6	0.4	N.D.	1.0	Trace

Disaccharide compositions (%) of untreated and fractionated CS were determined by HPLC on an amino-bound silica column after chondroitinase ABC digestion as described previously. Di-0S, ^4,5HexA(l-3)GalNAc; Di-4S, ^4,5HexA(l-3)GalNAc(4-O-sulfate); Di-6S, ^4,5HexA(l-3)GalNAc(6-O-sulfate); Di-diSE, ^4,5HexA(l-3)GalNAc(4,6-O-disulfate); Di-diSB, ^4,5HexA(2-O-sulfate)(l-3)GalNAc(4-O-sulfate); Di-diSD, ^4,5HexA(2-O-sulfate)(l-3)GalNAc(4-O-sulfate); Di-tirS, ^4,5HexA(2-O-sulfate)(l-3)GalNAc(4,6-O-disulfate); N.D.: not determined.