p21^WAF1/CIP1 Expression is Differentially Regulated by Metformin and Rapamycin

Figure 3

Metformin does not induce cell cycle arrest in AMPKα2-deficient cells. (a) Representative western blot image showing the effect of AMPKα2 knockdown, nonsilencing control (NS), knockdown (KD). (b) Densitometry analysis of P-AMPKα expression (mean ± SEM, ^∗𝑃<0.05). (c) Flow cytometry panels representing the effect of AMPKα2 knockdown on cell cycle arrest induced by 8 mM metformin (Met), compared with control (Ctrl). (d) High glucose treatment is comparable to AMPKα2-deficiency. The cells were cultured in 5.5 mM NG, 25 mM D-glucose (HG), for 48 h. For the last 24 h, 8 mM metformin (Met) was added in the indicated conditions. Data expressed as mean + SEM. NS 𝑃>0.05; ^∗∗𝑃<0.01, compared with Ctrl.

(a)

(b)

(c)

(d)