Novel Production Method for Plant Polyphenol from Livestock Excrement Using Subcritical Water Reaction
Figure 3
Quantitative HPLC analysis of polyphenols extracted
from the cattle excrement at 150, 200, 250 and C. (a) protocatechuic acid, (b) vanillic
acid, (c) syringic acid, and (d) vanillin. The circles show the yield of plant polyphenol per 1 g of
excrement. A reversed-phase
column (Shimadzu Shim-Pack CLC-ODS; = 6.0 mm; column length, 150 mm) was used at
the temperature of C. An aqueous solution of 2902.95 mL , 31.5 mL methanol, and 5.55 mL propan-2-ol, 60 mL acetic acid, 8.17 g sodium acetate
was used as a mobile phase. UV absorbance was detected at 280 nm.
