Table of Contents Author Guidelines Submit a Manuscript
International Journal of Chemical Engineering
Volume 2010, Article ID 162504, 6 pages
Research Article

Production of Ligninolytic Enzymes by White-Rot Fungus Datronia sp. KAPI0039 and Their Application for Reactive Dye Removal

1Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, 50 Chatuchak, Bangkok 10900, Thailand
2College of Environment, Kasetsart University, 50, Phaholyothin Road, Chatuchak, Bangkok 10900, Thailand
3Faculty of Agro-Industry, Kasetsart University, 50, Phaholyothin Road, Chatuchak, Bangkok 10900, Thailand

Received 4 December 2009; Accepted 25 June 2010

Academic Editor: Yves Andrès

Copyright © 2010 Pilanee Vaithanomsat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study focused on decolorization of 2 reactive dyes; Reactive Blue 19 (RBBR) and Reactive Black 5 (RB5), by selected white-rot fungus Datronia sp. KAPI0039. The effects of reactive dye concentration, fungal inoculum size as well as pH were studied. Samples were periodically collected for the measurement of color unit, Laccase (Lac), Manganese Peroxidase (MnP), and Lignin Peroxidase (LiP) activity. Eighty-six percent of 1,000  RBBR decolorization was achieved by 2% (w/v) Datronia sp. KAPI0039 at pH 5. The highest Lac activity (759.81  ) was detected in the optimal condition. For RB5, Datronia sp. KAPI0039 efficiently performed (88.01% decolorization) at 2% (w/v) fungal inoculum size for the reduction of 600  RB5 under pH 5. The highest Lac activity (178.57  ) was detected, whereas the activity of MnP and LiP was absent during this hour. The result, therefore, indicated that Datronia sp. KAPI0039 was obviously able to breakdown both reactive dyes, and Lac was considered as a major lignin-degradation enzyme in this reaction.