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International Journal of Chemical Engineering
Volume 2010 (2010), Article ID 391632, 11 pages
Review Article

Chlorophyll Extraction from Microalgae: A Review on the Process Engineering Aspects

Bio Engineering Laboratory (BEL), Department of Chemical Engineering, Monash University, Victoria 3800, Australia

Received 1 February 2010; Accepted 30 March 2010

Academic Editor: Ravichandra Potumarthi

Copyright © 2010 Aris Hosikian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chlorophyll is an essential compound in many everyday products. It is used not only as an additive in pharmaceutical and cosmetic products but also as a natural food colouring agent. Additionally, it has antioxidant and antimutagenic properties. This review discusses the process engineering of chlorophyll extraction from microalgae. Different chlorophyll extraction methods and chlorophyll purification techniques are evaluated. Our preliminary analysis suggests supercritical fluid extraction to be superior to organic solvent extraction. When compared to spectroscopic technique, high performance liquid chromatography was shown to be more accurate and sensitive for chlorophyll analysis. Finally, through capture and wastewater treatment, microalgae cultivation process was shown to have strong potential for mitigation of environmental impacts.