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International Journal of Chemical Engineering
Volume 2010, Article ID 490240, 6 pages
Research Article

Seasonal and Spatial Changes of Microorganism Communities in Constructed Wetlands: A Community Level Physiological Profiling Analysis

1Ecole des Mines de Nantes, GEPEA UMR CNRS 6144, 4, rue Alfred Kastler, B.P. 20722, 44307 Nantes Cedex, France
2Institut de Recherche en Biologie Végétale, Université de Montréal, 4101 rue Sherbrooke Est, Montréal QC, Canada H1X 2B2
3Laboratoire Optimisation de la Conception et Ingénierie de l'Environnement, Université de Savoie, Campus Scientifique, 73 376 Le Bourget du Lac Cedex, France

Received 15 January 2010; Accepted 22 March 2010

Academic Editor: Yves Andrès

Copyright © 2010 Florent Chazarenc et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In constructed wetlands, microorganisms associated with plants are assumed to play a major role. A one-year survey was conducted in five vertical flow constructed wetland systems that had been operating from 2 months to 8 years in small French villages (100–500 People Equivalent) to provide a better understanding of microbiological activity. The objective of our study was to highlight the most important factor generating variability between microorganisms communities compared to treatment performances. Results of community level physiological profiling using Biolog Ecoplates were analyzed using principal component analysis. The greatest microbial activity was observed in the oldest wetland during summer. Profiles of fed and rest bed were differentiated by the nature of the main carbon source metabolized. Whereas carbohydrates and carboxylic acids appeared to be better assimilated with fed beds, it seemed that phosphate compounds as well as amines allowed better growth in the plates inoculated with samples of rest beds. In all fed beds, the most important parameters affecting the diversity were the season and the age of the wetlands. There were only slight profile differences between surface and subsurface samples and between the first and second stage samples.