Research Article
Optimization of Process Parameters by Statistical Experimental Designs for the Production of Naringinase Enzyme by Marine Fungi
Table 2
Twenty trials Plackett-Burman experimental design with the response.
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Trials | Physical variables | Carbon sources | Nitrogen sources | Energy source | Metal ions |
Response enzyme activity (U/mL) | Moisture content | Weight of waste | Initial pH | Glucose | Grape fruit | Sucrose | Starch | Molasses | Peptone | Tryptone | Yeast extract | Soya bean | NaNO3 | (NH4)2PO4 | KH2PO4 | MgSO4 | MnSO4 | CuSO4 | FeSO4 | Experimental | Predicted |
| 1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | 0.04 | 0.02 | 2 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | 1.43 | 1.40 | 3 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | 4.16 | 4.19 | 4 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | 3.12 | 3.15 | 5 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | 1.56 | 1.58 | 6 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | 0.08 | 0.09 | 7 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | 1.95 | 1.97 | 8 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | 3.25 | 3.22 | 9 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | 0.65 | 0.63 | 10 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | 3.38 | 3.36 | 11 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | 2.34 | 2.31 | 12 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | 2.08 | 2.06 | 13 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | −1 | 0.26 | 0.29 | 14 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | 1.69 | 1.66 | 15 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | 9.10 | 9.13 | 16 | −1 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | 5.72 | 5.70 | 17 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | 1.63 | 1.64 | 18 | +1 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | 3.51 | 3.54 | 19 | +1 | +1 | +1 | −1 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | 11.70 | 11.67 | 20 | +1 | −1 | +1 | −1 | −1 | −1 | −1 | +1 | +1 | −1 | +1 | +1 | −1 | −1 | +1 | +1 | +1 | +1 | −1 | 6.50 | 6.52 |
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