Nile Red Staining for Oil Determination in Microalgal Cells: A New Insight through Statistical Modelling

Figure 4

Fluorescence intensity predicted by the quadratic model versus fluorescence intensity observed in the experiments. Refer to Table 2 for experimental conditions and observed fluorescence intensity values. Refer to Table 3 for regression coefficients of the quadratic models. Fluorescence intensity was determined at excitation wavelength of 530 nm and emission wavelength of 607 nm.