Nile Red Staining for Oil Determination in Microalgal Cells: A New Insight through Statistical Modelling

Figure 5

3D response surface plots of fluorescence intensity as predicted by the quadratic model. In each plot, the effects of two independent variables (in coded values) on fluorescence intensity are simultaneously demonstrated. For each surface plot, the other independent variables which do not form the plot axes are fixed (in coded values): (a) incubation temperature = 0, Nile red dye concentration = 0; (b) microalgal cell concentration = 1, ultrasonication power = 0. Excitation wavelength = 530 nm, emission wavelength = 607 nm, and poststaining time = 15 min.

(a)

(b)