Nile Red Staining for Oil Determination in Microalgal Cells: A New Insight through Statistical Modelling

Table 1

Real and coded values of the independent variables investigated in the factorial experimental design.

Microalgal cell concentration	Real value (g dried microalgae/L culture)		0	0.11
	Coded value		0	1
Ultrasonication power	Real value (W)	0	65	130
	Coded value	−1	0	1
Incubation temperature	Real value (°C)	20	30	40
	Coded value	−1	0	1
Nile red dye concentration	Real value (µg dye/mL culture)	0	2.5		6.25
	Coded value	−1	0		1.5