Research Article
One-Step Ultrafiltration Process for Separation and Purification of a Keratinolytic Protease Produced with Feather Meal
Table 1
Experimental design 23 in coded levels (real values).
| | Trial | MWCO (kDa) | pH | Pressure (MPa) |
| | 1 | −1 (10) | −1 (7.0) | −1 (0.147) | | 2 | +1 (30) | −1 (7.0) | −1 (0.147) | | 3 | −1 (10) | +1 (8.0) | −1 (0.147) | | 4 | +1 (30) | +1 (8.0) | −1 (0.147) | | 5 | −1 (10) | −1 (7.0) | +1 (0.245) | | 6 | +1 (30) | −1 (7.0) | +1 (0.245) | | 7 | −1 (10) | +1 (8.0) | +1 (0.245) | | 8 | +1 (30) | +1 (8.0) | +1 (0.245) |
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MWCO: molecular weight cutoff.
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