Research Article
Optimization of Deep Eutectic Solvents Extraction of Effective Components from Phellodendron chinense Schneid by Response Surface Methodology
Table 2
Results of recovery test of two active constituents.
| | Constituent | Original amount (μg) | Added amount (μg) | Total measured amount (μg) | Recovery rate (%) | Average recovery (%) | RSD (%) |
| | Palmatine | 140.220 | 139.000 | 277.346 | 98.65 | 99.31 | 1.94 | | 142.402 | 139.000 | 278.261 | 97.74 | | | | 140.766 | 139.000 | 282.002 | 101.61 | | | | 127.671 | 139.000 | 269.254 | 101.86 | | | | 136.401 | 139.000 | 271.942 | 97.51 | | | | 143.494 | 139.000 | 280.359 | 98.46 | | |
| | Berberine | 417.103 | 416.000 | 828.205 | 98.82 | 98.89 | 1.12 | | 423.595 | 416.000 | 828.994 | 97.45 | | | | 418.726 | 416.000 | 836.081 | 100.33 | | | | 379.775 | 416.000 | 794.346 | 99.66 | | | | 405.742 | 416.000 | 812.502 | 97.78 | | | | 426.841 | 416.000 | 840.002 | 99.32 | | |
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The above results showed that the HPLC analysis method was accurate and reproducible.
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