International Journal of Dentistry

International Journal of Dentistry / 2009 / Article

Case Report | Open Access

Volume 2009 |Article ID 654239 |

Sergio Matarasso, Vincenzo Daniele, Vincenzo Iorio Siciliano, Michele D. Mignogna, Gianmaria Andreuccetti, Carlo Cafiero, "The Effect of Recombinant Granulocyte Colony-Stimulating Factor on Oral and Periodontal Manifestations in a Patient with Cyclic Neutropenia: A Case Report", International Journal of Dentistry, vol. 2009, Article ID 654239, 6 pages, 2009.

The Effect of Recombinant Granulocyte Colony-Stimulating Factor on Oral and Periodontal Manifestations in a Patient with Cyclic Neutropenia: A Case Report

Academic Editor: Jasim M. Albandar
Received04 May 2009
Revised28 Jul 2009
Accepted30 Nov 2009
Published18 Feb 2010


Cyclic Neutropenia (CN) is characterized by recurrent infections, fever, oral ulcerations, and severe periodontitis as result of the reduced host defences. The previous studies have established the effectiveness of recombinant granulocyte colony-stimulating factor (GCSF) to increase the number and the function of neutrophils in the peripheral blood in this disease. In a 20-year-old Caucasian female with a diagnosis of cyclic neutropenia, oral clinical examination revealed multiple painful ulcerations of the oral mucosa, poor oral hygiene conditions, marginal gingivitis, and moderate periodontitis. The patient received a treatment with G-CSF (Pegfilgrastim, 6 mg/month) in order to improve her immunological status. Once a month nonsurgical periodontal treatment was carefully performed when absolute neutrophil count (ANC) was 500/ L. The treatment with G-CSF resulted in a rapid increase of circulating neutrophils that, despite its short duration, leaded to a reduction in infection related events and the resolution of the multiple oral ulcerations. The disappearance of oral pain allowed an efficacy nonsurgical treatment and a normal tooth brushing that determined a reduction of probing depth (  mm) and an improvement of the oral hygiene conditions recorded at 6-month follow-up.

1. Introduction

Neutrophils play a critical role in the host defence mechanism against bacterial infections [1]. There are 3 general guidelines used to classify theseverity of neutropenia based on the absolute neutrophil count (ANC) measured in cells per microliter of blood:

(i)mild neutropenia (1000 ANC 1500)—minimal risk of infection,(ii)moderate neutropenia (500 ANC 1000)—moderate risk of infection,(iii)severe neutropenia (ANC 500)—severe risk of infection.

The above mentioned ranges were developed in Caucasians. In colored population, mild neutropenia is a normal phenomenon, and neutropenia in this population is more properly defined as ANC 1200. Higher cutoffs may lead to overdiagnosis of neutropenia in the colored population [2]. The clinical consequence of the disease is an increased infective diathesis proportional to the severity of the neutropenia. Several forms of neutropenia (agranulocytosis, familiar benign neutropenia, severe chronic neutropenia) can be found associated with oral manifestations. Severe chronic neutropenia is characterized by a selective decrease in circulating neutrophils and includes a heterogeneous group of haematological diseases divided into three main syndromes: idiopathic neutropenia, congenital forms of neutropenia, and cyclic neutropenia.

The present paper is aimed at evaluating the efficacy of a non surgical periodontal treatment associated with G-CSF therapy in a patient suffering from cyclic neutropenia (CN) associated with oral manifestations and periodontal disease. Written informed consent was obtained by the patient and the authors declare no conflict of interest related to this paper.

2. Case Presentation

A 20-year-old Caucasian female with diagnosis of CN was referred by the Department of Immunology and Allergology, to the Department of Periodontology of the University of Naples Federico II, Italy. Anamnesis revealed that since the first year of life the patient has been suffering from repeated phases of fever and oral ulcerations, associated with pain and lymphadenopathy. During childhood and adolescence the patient had repeated admissions to the hospital, but CN was diagnosed only in September 2007. The haematological findings (complete laboratory work-up) showed the neutropenic phase recurred approximately every 21 days. The lowest neutrophil count was /μL. A light enlargement of submandibular and cervical lymph nodes was revealed by palpation and moreover fever ( C) sinusitis and pharyngitis were recorded during the neutropenic phase. In addition she reported to brush her teeth three times a day but during the neutropenic crisis oral hygiene was hindered by pain due to oropharyngeal ulcerations (Figures 1, 2, and 3). Full Mouth Plaque Score (FMPS) and Full Mouth Bleeding Score (FMBS), recorded on 6 sites per tooth, were 66.7% and 64.8%, respectively (Figure 4). Periodontal examination revealed a generalized increased probing depths (Figure 5). No furcations were involved and no gingival recessions were recorded. Tooth mobility was not present. An X-ray examination revealed bone loss on interproximal aspect of maxillary and mandibular teeth (Figure 6). A diagnosis of moderate periodontitis as manifestation of systemic disease was made.

Since September 2007 up till February 2008 the patient received a systemic treatment with Prednisolone 10 mg/die o.s./7 days (Deltacortene, Bruno Farmaceutici) during the neutropenia phases. The above therapy had a minimum effect on severity of oral ulcerations and produced minimal improvement in ANC. At this regard pain and burning determined poor oral hygiene conditions ( % and %).

In March 2008, the patient received a new treatment with GCSF administration consisting of subcutaneous injection of Pegfilgrastim 6 mg (Neupopeg, Dompè Biotec S.p.A., Italy) once a month.

Periodontal treatment including oral hygiene instructions, scaling, root planning, and polishing was meticulously carried out once a month when absolute neutrophil count (ANC) was 1500/μL and for this reason prophylactic antibiotic therapy was not prescribed. During neutropenic cycles the patient was advised to rinse her mouth twice a day with 0.2% chlorhexidine gluconate. A very high level of compliance was recorded. A complete laboratory work-up was performed every week. Therapy with G-CSF initially led to a “peak” of WBC and ANC and subsequently to a normalization for few days (Table 1). No side effects has been reported by the patient during the treatment and no adverse effects were noted when the counts were greater than /μL. A reduction of general and oral manifestations during the neutropenic cycles was constantly recorded. One week after neutropenic phase periodontal re-evaluation was carried out in order to assess the plaque control and the tissutal response to the treatment.

( / L)( / L)( / L)( / L)


21/04/2008 Subcutaneous injection of G-CSF


30/05/2008 Subcutaneous injection of G-CSF


28/06/2008 Subcutaneous injection of G-CSF


28/07/2008 Subcutaneous injection of G-CSF


30/08/2008 Subcutaneous injection of G-CSF


WBC: White blood cell count; Neutr: Neutrophil; Limph: Lymphocytes; Mono: Monocytes.

A considerable improvement of oral hygiene conditions was observed: FMPS and FMBS remained constantly 25% and a period general reduction of probing depth (  mm) during the successive 6-month follow-up was recorded (Figures 7 and 8). Microbiological analysis was performed. Microbiological sampling included selection of the deepest periodontal pocket in each quadrant of the dentition based on the probing depth measurements. Sample sites were isolated with cotton rolls and supragingival plaque was carefully removed with curettes and cotton pallets. Subsequently, two paper points were inserted to the depth of the pocket and left in place for 10 seconds.

The paper points were transferred to a vial to be send to the microbiological laboratory in order to perform Polymerase Chain Reaction (PCR, Lab Oral International, The Netherlands). Microbiological analysis revealed no residual pathogenic subgingival microflora but an elevated level of Fusobacterium nucleatum.

3. Discussion

Cyclic neutropenia (CN) is a chronic rare disorder in the production of the neutrophils, presenting at about 19–21 days intervals. It is clinically characterized by a periodic decrease in the circulating neutrophil numbers determining recurrent infections.

CN is transmitted by autosomal dominant gene and occurs in infancy but occasionally in adult life [3]. CN is identified as an intrinsic defect of granulocyopoiesis, whose molecular alteration resides in the gene of neutrophil elastase (ELA 2) located on the chromosome 19p13.3.

In the early maturation stage, the mutation of the gene ELA2 led to an intramedullary apoptosis of neutrophils, that causes regular phases of neutropenia in peripheral blood [4]. The neutropenic phases are typically reported to fall in the range of 19 to 21 days, although recent data indicate that longer periods occur in some patients. The episodes last 5–8 days [5]. The diagnostic criterion of CN is absolute neutrophil count (ANC) less than /μL, at least 3 to 5 consecutive days per cycle, for each of three regularly spaced cycles.

As a result of neutropenic phases, haematological findings could reveal increasing in the number of platelets, monocytes, lymphocytes, eosinophils, and reticulocytes [6]. Neutropenia predisposes to respiratory or muco-cutaneous bacterial infection and in some cases was reported in association with otitis media and upper respiratory infection [7]. These manifestations are similar, but less severe, to those commonly reported by patients suffering from agranulocytosis. A general malaise, fever, lymphadenopathy, and oral ulcerations are very common findings during neutropenic periods [8]. Severe periodontitis and oral ulcerations have been reported in the majority of the recorded cases, meanwhile ulcerations may be the unique oral manifestation in about 20% of patients [911]. The mucosal lesions can affect any part of the oral mucosa during the neutropenic phases and cause pain and very intense burning that could discourage the patient to maintain a good oral hygiene. Gingivitis is common in CN and severe periodontitis may affect both deciduous and permanent dentitions [12]. In the international literature, severe periodontitis is constantly recorded in subject suffering from CN [1316], meanwhile this case report is characterized by few periodontal lesions demonstrating that a significant decrease of neutrophil polymorphonucleate leukocytes could be associated with moderate periodontal lesions. Medical management of neutropenias is mainly symptomatic and consists of aggressive antibiotic treatment of febrile patients suspected of having bacterial infections. Other therapies of uncertain efficacy include glucocorticoids, androgenic steroids, immunoglobulins, and plasmapheresis [1721]. In the last years therapeutic protocols based on the use of recombinant granulocyte colony-stimulating factor (G-CSF) [2225] that increases for few days the number and the function of neutrophils in the peripheral blood have been proposed by several authors [2629]. Filgrastim is a granulocyte colony-stimulating factor (G-CSF) analog used to stimulate the proliferation and differentiation of granulocytes. It is produced by recombinant DNA technology and is only slightly different from G-CSF naturally made in humans. Pegfilgrastim is a pegylated form of Filgrastim: pegylation leads to prolongation of its half-life without loss of activity [22, 23]. The recent data demonstrate that Pegfilgrastim, at doses ranging from 6 to 12 mg alone or together with chemotherapy, is capable of efficiently mobilizing peripheral blood stem cells, thus resulting in rapid reconstitution of haematopoiesis. According to data present in the literature, in which no clinically meaningful difference between a single monthly dose of Pegfilgrastim (6 mg) and multiple daily doses of Filgrastim (5  g/kg/die) was recorded, a single dose of G-CSF (Pegfilgrastim) was monthly administered to the patient [3033], but there are no organized trials to test or compare Pegfilgrastim versus Filgrastim or other types of G-CSF for treatment of cyclic or chronic neutropenia.

The monthly therapy with G-CSF caused a rapid increase of circulating neutrophils that, even if of short duration, determined the disappearance of oral ulcerations during the neutropenic phases. These results confirm the outcomes found in the literature, in which the use of G-CSF determined a reduction of oral ulcerations in patient suffering from CN [20]. Moreover the disappearance of ulcerations determined an enhancement of the compliance of the patient who meticulously followed the oral care program.

At 6-month follow-up nonsurgical treatment of the periodontitis determined a general reduction of probing depth (  mm) and a proper tooth brushing leads to an improvement of oral hygiene resulting in an effective plaque control by the patient ( ; ). At baseline the patient refused to undergo to microbiological analysis and accepted at 6-month follow-up. At 6-month follow-up microbiological analysis revealed no residual pathogenic subgingival microflora but an elevated level of Fusobacterium nucleatum.

Fusobacterium nucleatum is an anaerobic Gram-negative nonsporeforming oral bacterium found in the normal flora of the mouth, that plays a role in periodontal disease. Because of its opportunistic nature, Fusobacterium nucleatum does not affect the environment directly and is not considered a major dental pathogen on its own although it can adhere to a wide range of other organisms, such as Porphyromonas gingivalis, and contribute to the development of periodontitis. On the basis of the present microbiological results, a systemic antibiotic therapy was not prescribed to the patient. In conclusion the present results showed that the therapy with recombinant granulocyte colony-stimulating factor (G-CSF) allowed the oral mucosa to heal and influenced the entity of neutropenic cycle. Moreover the periodontal treatment is effective in a patient with CN when compliance is sufficient high to follow the maintenance care program.


The authors thank Professor Massimo Triggiani and Professor Angela Petraroli, Department of Immunology and Allergology of the University of Naples Federico II, for the medical management of the present case.


  1. A. W. Segal, “How neutrophils kill microbes,” Annual Review of Immunology, vol. 23, pp. 197–223, 2005. View at: Publisher Site | Google Scholar
  2. M. M. Hsieh, J. E. Everhart, D. D. Byrd-Holt, J. F. Tisdale, and G. P. Rodgers, “Prevalence of neutropenia in the U.S. population: age, sex, smoking status, and ethnic differences,” Annals of Internal Medicine, vol. 146, no. 7, pp. 486–492, 2007. View at: Google Scholar
  3. Y. Nakai, C. Ishihara, S. Ogata, and T. Shimono, “Oral manifestations of cyclic neutropenia in a Japanese child: case report with a 5-year follow-up,” Pediatric Dentistry, vol. 25, no. 4, pp. 383–388, 2003. View at: Google Scholar
  4. C. Bellanné-Chantelot, S. Clauin, T. Leblanc et al., “Mutations in the ELA2 gene correlate with more severe expression of neutropenia: a study of 81 patients from the French Neutropenia Register,” Blood, vol. 103, no. 11, pp. 4119–4125, 2004. View at: Publisher Site | Google Scholar
  5. C. Haurie, D. C. Dale, and M. C. Mackey, “Cyclical neutropenia and other periodic hematological disorders: a review of mechanisms and mathematical models,” Blood, vol. 92, no. 8, pp. 2629–2640, 1998. View at: Google Scholar
  6. M. C. Dinauer, J. A. Lekstrom-Himes, and D. C. Dale, “Inherited neutrophil disorders: molecular basis and new therapies,” Hematology, pp. 303–318, 2000. View at: Google Scholar
  7. J. F. Prichard, D. M. Ferguson, J. Windmiller, and W. C. Hurt, “Prepubertal periodontitis affecting the deciduous and permanent dentition in a patient with cyclic neutropenia. A case report and discussion,” Journal of Periodontology, vol. 55, no. 2, pp. 114–122, 1984. View at: Google Scholar
  8. C. Scully, E. MacFadyen, and A. Campbell, “Oral manifestations in cyclic neutropenia,” British Journal of Oral Surgery, vol. 20, no. 2, pp. 96–101, 1982. View at: Google Scholar
  9. L. M. Long Jr., J. R. Jacoway, and J. W. Bawden, “Cyclic neutropenia: case report of two siblings,” Pediatric Dentistry, vol. 5, no. 2, pp. 142–144, 1983. View at: Google Scholar
  10. P. Spencer and J. E. Fleming, “Cyclic neutropenia: a literature review and report of case,” ASDC Journal of Dentistry for Children, vol. 52, no. 2, pp. 108–113, 1985. View at: Google Scholar
  11. N. Yamalik, E. Yavuzyilmaz, F. Cağlayan et al., “Periodical gingival bleeding as a presenting symptom of periodontitis due to underlying cyclic neutropenia. Case report,” Australian Dental Journal, vol. 38, no. 4, pp. 272–276, 1993. View at: Google Scholar
  12. H. Rylander and I. Ericsson, “Manifestations and treatment of periodontal disease in a patient suffering from cyclic neutropenia,” Journal of Clinical Periodontology, vol. 8, no. 2, pp. 77–87, 1981. View at: Google Scholar
  13. I. Brodsky, H. A. Reimann, and L. H. Dennis, “Treatment of cyclic neutropenia with testosterone,” The American Journal of Medicine, vol. 38, no. 5, pp. 802–806, 1965. View at: Google Scholar
  14. D. G. Wright, A. S. Fauci, D. C. Dale, and S. M. Wolff, “Correction of human cyclic neutropenia with prednisolone,” The New England Journal of Medicine, vol. 298, no. 6, pp. 295–300, 1978. View at: Google Scholar
  15. K. J. Roozendaal, K. A. Dicke, and M. L. B. Flaes, “Effect of oxymetholone on human cyclic haematopoiesis,” British Journal of Haematology, vol. 47, no. 2, pp. 185–193, 1981. View at: Google Scholar
  16. D. S. Verma, G. Spitzer, A. R. Zander, K. A. Dicke, and K. B. McCredie, “Cyclic neutropenia and T lymphocyte suppression of granulopoiesis: abrogation of the neutropenic cycles by lithium carbonate,” Leukemia Research, vol. 6, no. 4, pp. 567–576, 1982. View at: Google Scholar
  17. G. K. Von Schulthess, J. Fehr, and C. Dahinden, “Cyclic neutropenia: amplification of granulocyte oscillations by lithium and long-term suppression of cycling by plasmapheresis,” Blood, vol. 62, no. 2, pp. 320–326, 1983. View at: Google Scholar
  18. B. I. Lord, L. B. Woolford, and G. Molineux, “Kinetics of neutrophil production in normal and neutropenic animals during the response to filgrastim (r-metHu G-CSF) or filgrastim SD/01 (PEG-r-metHu G-CSF),” Clinical Cancer Research, vol. 7, no. 7, pp. 2085–2090, 2001. View at: Google Scholar
  19. G. Molineux, “The design and development of pegfilgrastim (PEG-rmetHuG-CSF, Neulasta),” Current Pharmaceutical Design, vol. 10, no. 11, pp. 1235–1244, 2004. View at: Publisher Site | Google Scholar
  20. D. C. Dale, M. A. Bonilla, M. W. Davis et al., “A randomized controlled phase III trial of recombinant human granulocyte colony-stimulating factor (filgrastim) for treatment of severe chronic neutropenia,” Blood, vol. 81, no. 10, pp. 2496–2502, 1993. View at: Google Scholar
  21. G. Simon and L. Maródi, “Successful treatment of cyclic neutropenia associated with hyperimmunoglobulin M syndrome using recombinant granulocyte-colony stimulating factor,” Orvosi Hetilap, vol. 136, no. 40, pp. 2169–2172, 1995. View at: Google Scholar
  22. W. Bensinger, J. Singer, F. Appelbaum et al., “Autologous transplantation with peripheral blood mononuclear cells collected after administration of recombinant granulocyte stimulating factor,” Blood, vol. 81, no. 11, pp. 3158–3163, 1993. View at: Google Scholar
  23. S. Kitagawa, A. Yuo, L. M. Souza, M. Saito, Y. Miura, and F. Takaku, “Recombinant human granulocyte colony-stimulating factor enhances superoxide release in human granulocytes stimulated by the chemotactic peptide,” Biochemical and Biophysical Research Communications, vol. 144, no. 3, pp. 1143–1146, 1987. View at: Google Scholar
  24. A. Yuo, S. Kitagawa, A. Ohsaka et al., “Recombinant human granulocyte colony-stimulating factor as an activator of human granulocytes: potentiation of responses triggered by receptor-mediated agonists an stimulation of C3bi receptor expression and adherence,” Blood, vol. 74, no. 6, pp. 2144–2149, 1989. View at: Google Scholar
  25. R. Repp, T. Valerius, A. Sendler et al., “Neutrophils express the high affinity receptor for IgG (FcγRI, CD64) after in vivo application of recombinant human granulocyte colony-stimulating factor,” Blood, vol. 78, no. 4, pp. 885–889, 1991. View at: Google Scholar
  26. P. Holmstrup and M. Glick, “Treatment of periodontal disease in the immunodeficient patient,” Periodontology 2000, vol. 28, no. 1, pp. 190–205, 2002. View at: Google Scholar
  27. P. N. Baer and V. J. Iacono, “Cyclic neutropenia: report of a case with a 15-year follow up,” Periodontal Clinical Investigations, vol. 16, no. 1, pp. 14–19, 1994. View at: Google Scholar
  28. R. J. Genco, “Current view of risk factors for periodontal diseases,” Journal of Periodontology, vol. 67, supplement 10, pp. 1041–1049, 1996. View at: Google Scholar
  29. M. A. da Fonseca and F. Fontes, “Early tooth loss due to cyclic neutropenia: long-term follow-up of one patient,” Special Care in Dentistry, vol. 20, no. 5, pp. 187–190, 2000. View at: Google Scholar
  30. F. A. Holmes, S. E. Jones, J. O'Shaughnessy et al., “Comparable efficacy and safety profiles of once-per-cycle pegfilgrastim and daily injection filgrastim in chemotherapy-induced neutropenia: a multicenter dose-finding study in women with breast cancer,” Annals of Oncology, vol. 13, no. 6, pp. 903–909, 2002. View at: Publisher Site | Google Scholar
  31. M. D. Green, H. Koelbl, J. Baselga et al., “A randomized double-blind multicenter phase III study of fixed-dose single-administration pegfilgrastim versus daily filgrastim in patients receiving myelosuppressive chemotherapy,” Annals of Oncology, vol. 14, no. 1, pp. 29–35, 2003. View at: Publisher Site | Google Scholar
  32. J. Sierra, J. Szer, J. Kassis et al., “A single dose of pegfilgrastim compared with daily filgrastim for supporting neutrophil recovery in patients treated for low-to-intermediate risk acute myeloid leukemia: results from a randomized, double-blind, phase 2 trial,” BMC Cancer, vol. 8, article 195, 2008. View at: Publisher Site | Google Scholar
  33. N. Russell, R. Mesters, J. Schubert et al., “A phase 2 pilot study of pegfilgrastim and filgrastim for mobilizing peripheral blood progenitor cells in patients with non-Hodgkin's lymphoma receiving chemotherapy,” Haematologica, vol. 93, no. 3, pp. 405–412, 2008. View at: Publisher Site | Google Scholar

Copyright © 2009 Sergio Matarasso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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