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International Journal of Dentistry
Volume 2010 (2010), Article ID 275103, 6 pages
http://dx.doi.org/10.1155/2010/275103
Research Article

Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

1Department of Dental Hygiene, Fukuoka College of Health Sciences, 2-15-1 Tamura, Sawara-Ku, Fukuoka 814-0193, Japan
2Fujimura Clinic, Fukuoka, Japan
3Hamadayama Smile Dental Clinic, Tokyo, Japan

Received 26 July 2010; Revised 29 September 2010; Accepted 7 October 2010

Academic Editor: Michael E. Razzoog

Copyright © 2010 Noriaki Kaneko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In order to establish a method of obtaining rat gingival mitochondria (Mt), Mt fractions were prepared in various combinations of homogenizing time with collagenase concentration. Rat gingival tissues were excised, minced, treated with collagenase, homogenized, and subjected to differential centrifugation rates. Both the respiratory control ratio (RCR) and adenosine diphosphate/oxygen (ADP/O) ratio of the Mt fraction prepared in a combination of 40, 50, or 60 sec homogenization with collagenase in a concentration range of 0.115%–0.130% (w/v) were measured. The values for the RCR and ADP/O ratio of the Mt fraction obtained in an optimal condition was 1 . 8 0 ± 0 . 0 5 and 1 . 6 5 ± 0 . 0 3 , respectively. These results suggest that Mt of fairly high quality can be obtained through this refined combination of the homogenizing time and collagenase concentration.