Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

Figure 2

A polarographic trace of gingival mitochondria (Mt). Mt were prepared from approximately 1.0 g rat gingival tissue using an optimized preparative procedure displaying apparent respiratory activity. The preparation conditions are 50 sec of polytron homogenizer treatment and 0.120% (w/v) collagenase. Oxygen consumption by the gingival Mt (0.5 mg protein/ 600   μL buffer) was measured with the addition of substrate (0.6 mM succinate) followed by 30   μM adenosine diphosphate (ADP) at 25°C.