Preparation of Rat Gingival Mitochondria with an Improved Isolation Method

Table 1

Specific activity of succinate dehydrogenase (SDH) in the various fractions in the process of mitochondrial isolation.

Fractions	Specific activity
	(U/mg protein)
S1	0.51 ± 0.05∗
S2	0.72 ± 0.05∗
S3	0.22 ± 0.02∗
N	0.25 ± 0.02∗
Mt	5.67 ± 0.32

S1, S2, and S3 designate the first, second, and third supernatant fractions in the process of mitochondrial isolation, respectively, while N and Mt designate the precipitated fractions of the nuclei and mitochondria, respectively, (see Figure 1). Enzymatic digestion was carried out under the conditions of 0.120% (w/v) collagenase for 20 min with 50 sec homogenization. Specific activity of succinate dehydrogenase was measured by the modified Slater method [12].
∗Significantly different compared with the mitochondrial (Mt) fraction, 𝑃<.05.