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International Journal of Dentistry
Volume 2010, Article ID 513461, 6 pages
Research Article

Rank Protein Immunolabeling during Bone-Implant Interface Healing Process

1Department of Surgery and General Clinical, Araçatuba Dental School, Universidade Estadual Paulista, UNESP, Rua José Bonifácio 1190, Bloco 10A, Vila Mendonça, 16015-050 Araçatuba, Brazil
2Department of Dental Materials and Prothesis, São José dos Campos Dental School, Universidade Estadual Paulista, UNESP, Brazil

Received 24 March 2010; Revised 18 June 2010; Accepted 18 June 2010

Academic Editor: A. B. M. Rabie

Copyright © 2010 Francisley Ávila Souza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The purpose of this paper was to evaluate the expression of RANK protein during bone-healing process around machined surface implants. Twenty male Wistar rats, 90 days old, after having had a 2 mm diameter and 6 mm long implant inserted in their right tibias, were evaluated at 7, 14, 21, and 42 days after healing. After obtaining the histological samples, slides were subjected to RANK immunostaining reaction. Results were quantitatively evaluated. Results. Immunolabeling analysis showed expressions of RANK in osteoclast and osteoblast lineage cells. The statistical analysis showed an increase in the expression of RANK in osteoblasts at 7 postoperative days and a gradual decrease during the chronology of the healing process demonstrated by mild cellular activity in the final stage ( ). Conclusion. RANK immunolabeling was observed especially in osteoclast and osteoblast cells in primary bone during the initial periods of bone-healing/implant interface.