(a) Principle of the Arcturus laser capture microdissection. A plastic film is covered over the specimen. When the plastic film is removed, the dissected tissue by IR laser is attached to the film and isolated from the rest of the sample section. (b) Principle of the Zeiss’s PALM microdissection. The tissue section has been mounted on a PEN foiled slide. Then, UV laser beam focused and cut a contour around the area of the target tissue via inverted microscope. The dissected tissue is collected by photonic pressure; using laser pressure to lift the dissected tissue into a collecting cap is named laser pressure catapulting. (c) Principle of the Leica AS LMD microdissection. The tissue section that has been mounted on a PEN foiled slide is set upside down of the stage. Then, laser beam dissect the target tissue. The dissected tissue falls into collecting cap positioned under the specimen.
