Step-by-step characterization of the technique based on LCM used for retrieval of either the odontoblastic layer or the tissue underneath the odontoblastic layer from formaldehyde-fixated paraffin embedded tissue sections that had been demineralized. (a) H&E staining of the engineered dental pulp-like tissue after 21 days of implantation. D: dentin. P: engineered dental pulp. Arrowheads: odontoblastic layer. (b) Air dried slide of the engineered dental pulp-like tissue. D: dentin. P: engineered dental pulp. (c) Removal of the tissue underneath the odontoblastic layer. (d) Retrieval of the odontoblastic layer.
