Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells
Figure 1
Phase-contrast micrographs. (a) Mixed culture of enamel organ-derived epithelial (EOE) cells and dental follicle cells after 1 week of culture. The epithelial cells (e) have formed an island within the strongly proliferating dental follicle cells (df). (b) EOE cells in LHC-9 medium. After replacing the serum-medium with LHC-9, only EOE cells survived in the nonserum medium. The colony of EOE cells showed a cobblestone appearance associated with typical epithelial morphology. (c) Subcultured EOE cells (passage 1) grown on the insulin-like growth factor-I/insulin-like growth factor binding protein-3/vitronectin complex after 10 days of cultivation. (d) EOE cells cultured on insulin-like growth factor-I/insulin-like growth factor binding protein-3/vitronectin (IGF-IGFBP-VN) complex. After 1 passaged, EOE cells had the same characteristic morphology. After 25 days of cultivation, the colony became confluent. The EOE cells had the same characteristic morphology. (e) EOE cells cultured on collagen type I-coated dishes. The cells showed a vague outline and expanded morphology. (f) EOE cells cultured on PS. The EOE cells displayed on extended morphology. Scale bars: 100 μm (a), 50 μm (b–d) length.
