Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells

Figure 3

Semiquantitative reverse transcription-polymerase chain reaction analysis of enamel organ-derived epithelial (EOE) cells cultured on insulin-like growth factor-I/insulin-like growth factor binding protein-3/vitronectin complex (IGF) in comparison with the mixed cells from the primary culture (P), and EOE cells on polystyrene (PS) and collagen- type-I-(Co)- coated dishes. Amelogenin (AML), ameloblastin (AMBL), enamelin (ENAM), matrix metalloprotease-20 (enamelysin, MMP20), collagen I (COLI), insulin-like growth factor-I (IGF-I), insulin-like growth factor I receptor (IGF-IR), and beta-actin (Actin) in cultured EOE cells were examined. Primary cells (P) expressed all the examined genes. Cultured EOE cells at passage 1 on PS expressed ameloblastin and enamelin. The expression pattern in the IGF culture was similar to that of the CO culture. Both IGF-I and IGF-IR were more strongly expressed in IGF compared to CO.