386282.fig.005a
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386282.fig.005b
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386282.fig.005c
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386282.fig.005d
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386282.fig.005e
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386282.fig.005f
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Figure 5: The implants at four weeks after transplantation. (a) Round-shaped implants were produced by the constructs formed between cultured enamel organ-derived epithelial (EOE) cells and dental pulp cells in a scaffold. (b) An enamel organ-like structure associated with an epithelial cell cluster was seen in the implant. Columnar cells were organized in a circle and dental pulp cells embraced the enamel organ. (c) Enamel-like tissue formation on the surface of the dentin, stained with hematoxylin-eosin. (d) High magnification of (c), showing that enamel-like tissue (black arrow) stained with eosin was strongly displayed in the implant. (e) High magnification of (c), showing that tall columnar ameloblast-like cells (am) were aligned perpendicular to the dentin-like tissues (d). (f) High magnification of (c), showing that amelogenin expression was strongly identified in the enamel-like tissue adjacent to the dentin-like tissues and ameloblast-like cells. Scale bars: 50 μm (b, d–f), 200 μm (c) length.