International Journal of Dentistry / 2012 / Article / Tab 1 / Research Article
Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells Table 1 Sequence of primer pairs used for semiquantitative RT-PCR.
Gene Sequence Annealing temperature (°C) Amplicon (bp) Accession number or reference Amelogenin Forward 5-CCTGCCTTTTGGGAGCA 50 328 NM213800 Reverse 5-TGGTGGTGTTGGGTTGGA Ameloblastin Forward 5-ATTCCCAACCTGGCAAGAGG 55 380 NM214037 Reverse 5-AGCGCTTTTAATGCCTTTGC Enamelin Forward 5-TGAGGAGATGATGCGCTATG 45 315 NM214241 Reverse 5-TGAGGTGTCTGGGTTTCCTC Enamelysin Forward 5-ATACGTGCAGCGAATAGATGC 45 290 NM213905 Reverse 5-CTATTTAGCAACCAATCCAGG Collagen type I Forward 5-GATCCTGCTGACGTGGCCAT 55 212 AY350905 Reverse 5-ACTCGTGCAGCCGTCGTAGA IGF-I Forward 5-GACGCTCTTCAGTTCGTGTG 50 348 NM214256 Reverse 5-ACTCGTGCAGAGCAAAGGAT IGF-IR Forward 5-ACTGTATGGTGGCCGAAGAC 50 391 NM214172 Reverse 5-ATCTCGTCCTTGATGCTGCT beta-actin Forward 5-TCGACCACAGGGTAGGTTTC 45 497 AF017079 Reverse 5-CCCCAGCATCAAAGGTAGAA
