Table 1: Sequence of primer pairs used for semiquantitative RT-PCR.

GeneSequenceAnnealing temperature (°C)Amplicon (bp)Accession number or reference

AmelogeninForward5-CCTGCCTTTTGGGAGCA50328NM213800
Reverse5-TGGTGGTGTTGGGTTGGA
AmeloblastinForward5-ATTCCCAACCTGGCAAGAGG55380NM214037
Reverse5-AGCGCTTTTAATGCCTTTGC
EnamelinForward5-TGAGGAGATGATGCGCTATG45315NM214241
Reverse5-TGAGGTGTCTGGGTTTCCTC
EnamelysinForward5-ATACGTGCAGCGAATAGATGC45290NM213905
Reverse5-CTATTTAGCAACCAATCCAGG
Collagen type IForward5-GATCCTGCTGACGTGGCCAT55212AY350905
Reverse5-ACTCGTGCAGCCGTCGTAGA
IGF-IForward5-GACGCTCTTCAGTTCGTGTG50348NM214256
Reverse5-ACTCGTGCAGAGCAAAGGAT
IGF-IRForward5-ACTGTATGGTGGCCGAAGAC50391NM214172
Reverse5-ATCTCGTCCTTGATGCTGCT
beta-actinForward5-TCGACCACAGGGTAGGTTTC45497AF017079
Reverse5-CCCCAGCATCAAAGGTAGAA