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International Journal of Dentistry
Volume 2012, Article ID 609689, 5 pages
http://dx.doi.org/10.1155/2012/609689
Clinical Study

Cultivable Anaerobic Microbiota of Infected Root Canals

1Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan
2Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan
3Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan
4Division of Pediatric Dentistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8514, Japan

Received 15 December 2011; Revised 30 January 2012; Accepted 5 February 2012

Academic Editor: Iris Slutzky-Goldberg

Copyright © 2012 Takuichi Sato et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. G. Sundqvist, Bacteriological Studies of Necrotic Pulps, Odontologisk Dissertations, Umeå University, Umeå, Sweden, 1976.
  2. T. Sato, E. Hoshino, H. Uematsu, and T. Noda, “Predominant obligate anaerobes in necrotic pulps of human deciduous teeth,” Microbial Ecology in Health and Disease, vol. 6, no. 6, pp. 269–275, 1993. View at Google Scholar · View at Scopus
  3. N. Ando and E. Hoshino, “Predominant obligate anaerobes invading the deep layers of root canal dentin,” International Endodontic Journal, vol. 23, no. 1, pp. 20–27, 1990. View at Google Scholar · View at Scopus
  4. Y. Ito, T. Sato, K. Yamaki et al., “Microflora profiling of infected root canal before and after treatment using culture-independent methods,” Journal of Microbiology, vol. 50, no. 1, pp. 58–62, 2012. View at Publisher · View at Google Scholar
  5. M. Sakamoto, J. F. Siqueira Jr., I. N. Rôças, and Y. Benno, “Bacterial reduction and persistence after endodontic treatment procedures,” Oral Microbiology and Immunology, vol. 22, no. 1, pp. 19–23, 2007. View at Publisher · View at Google Scholar · View at Scopus
  6. D. J. Lane, “16S/23S rRNA sequencing,” in Nucleic Acid Techniques in Bacterial Systematics, E. Stackebrandt and M. Goodfellow, Eds., John Wiley & Sons, Chichester, UK, 1991. View at Google Scholar
  7. J. R. Marchesi, T. Sato, A. J. Weightman et al., “Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA,” Applied and Environmental Microbiology, vol. 64, no. 12, pp. 795–799, 1998. View at Google Scholar · View at Scopus
  8. T. Sato, M. Sato, J. Matsuyama, and E. Hoshino, “PCR-restriction fragment length polymorphism analysis of genes coding for 16S rRNA in Veillonella spp,” International Journal of Systematic Bacteriology, vol. 47, no. 4, pp. 1268–1270, 1997. View at Google Scholar · View at Scopus
  9. T. Sato, J. Matsuyama, N. Takahashi et al., “Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length polymorphism,” Archives of Oral Biology, vol. 43, no. 3, pp. 247–252, 1998. View at Publisher · View at Google Scholar · View at Scopus
  10. T. Sato, M. Sato, J. Matsuyama, S. Kalfas, G. Sundqvist, and E. Hoshino, “Restriction fragment-length polymorphism analysis of 16S rDNA from oral asaccharolytic Eubacterium species amplified by polymerase chain reaction,” Oral Microbiology and Immunology, vol. 13, no. 1, pp. 23–29, 1998. View at Google Scholar · View at Scopus
  11. T. Sato and H. K. Kuramitsu, “Restriction fragment-length polymorphism analysis of 16S ribosomal RNA genes amplified by polymerase chain reaction for rapid identification of cultivable oral treponemes,” Oral Microbiology and Immunology, vol. 14, no. 2, pp. 117–121, 1999. View at Publisher · View at Google Scholar · View at Scopus
  12. T. Sato, J. Matsuyama, and N. Takahashi, “16S rRNA genes PCR-RFLP analysis for rapid identification of oral anaerobic gram-positive bacilli,” International Journal of Oral Biology, vol. 25, no. 3, pp. 87–91, 2000. View at Google Scholar
  13. T. Sato, J. P. Hu, K. Ohki et al., “Identification of mutans streptococci by restriction fragment length polymorphism analysis of polymerase chain reaction-amplified 16S ribosomal RNA genes,” Oral Microbiology and Immunology, vol. 18, no. 5, pp. 323–326, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. R. Sato, T. Sato, I. Takahashi, J. Sugawara, and N. Takahashi, “Profiling of bacterial flora in crevices around titanium orthodontic anchor plates,” Clinical Oral Implants Research, vol. 18, no. 1, pp. 21–26, 2007. View at Publisher · View at Google Scholar · View at Scopus
  15. K. Hashimoto, T. Sato, H. Shimauchi, and N. Takahashi, “Profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria,” American Journal of Dentistry, vol. 24, no. 5, pp. 295–299, 2011. View at Google Scholar
  16. E. Hoshino, “Predominant obligate anaerobes in human carious dentin,” Journal of Dental Research, vol. 64, no. 10, pp. 1195–1198, 1985. View at Google Scholar · View at Scopus
  17. H. Uematsu and E. Hoshino, “Predominant obligate anaerobes in human periodontal pockets,” Journal of Periodontal Research, vol. 27, no. 1, pp. 15–19, 1992. View at Google Scholar · View at Scopus
  18. L. Li, W. W. L. Hsiao, R. Nandakumar et al., “Analyzing endodontic infections by deep coverage pyrosequencing,” Journal of Dental Research, vol. 89, no. 9, pp. 980–984, 2010. View at Publisher · View at Google Scholar · View at Scopus
  19. J. F. Siqueira Jr., F. R. F. Alves, and I. N. Rôças, “Pyrosequencing analysis of the apical root canal microbiota,” Journal of Endodontics, vol. 37, no. 11, pp. 1499–1503, 2011. View at Google Scholar
  20. J. F. Siqueira Jr. and I. N. Rôçac, “Critical review in oral biology and medicine: diversity of endodontic microbiota revisited,” Journal of Dental Research, vol. 88, no. 11, pp. 969–981, 2009. View at Publisher · View at Google Scholar · View at Scopus