Porphyromonas gingivalis Outer Membrane Vesicles Mediate Coaggregation and Piggybacking of Treponema denticola and Lachnoanaerobaculum saburreum

Table 3

Effect of P. gingivalis outer membrane vesicles on the piggybacking of L. saburreum on T. denticola.

	Mean of cells ± S.D. (×10^3)
Assay	Number of bacteria in capillary tube*
	T. denticola	E. saburreum
T. denticola alone	71 ± 13 × 10^3	0
L. saburreum alone	0	3 ± 2 × 10^3
T. denticola + L. saburreum; (without vesicles)	66 ± 22 × 10^3	4 ± 3 × 10^3
T. denticola + L. saburreum; (with vesicles)	84 ± 26 × 10^3	20 ± 7 × 10^3†

* Determined using a Petroff-Hausser counting chamber.
^†Significant increase (𝑃 < 0.001) in the number of L. saburreum cells in the capillary tube.