Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
(a) Image registration of consecutive histological images in the -axis. (b) Sagittal cross-cut view through the center of an image stack comprising 108 serial sections, showing successful superimposition without marked irregularity or distortion in interior tissue geometry. Bar = 0.5 mm.((c)–(e)) Segmentation of cancer cells and Ki67-positive nuclei: (c) original double-immunostained image with DAB and Vector SG as chromogens; (d) segmentation of CK-positive cancer cell cytoplasm, shown as purple pseudocolor (note that Ki67-negative nuclei remained open within the segmented cytoplasm); and (e) stromal region (yellow) after subtraction of the tumor parenchyma (see text for details); (f) Ki67-positive nuclei (red) attained by ImageJ SG color segmentation. Bar = 50 μm.
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