Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front

Figure 3

(a, b) 3D view of the segmented tumor parenchymal (purple) and stromal (yellow) regions, respectively. (c) Enlargement of the CK-positive tumor architecture in the rectangle shown in (a), showing in detail the interconnected tumor texture and channels or holes corresponding to penetrating stromal strands. (d) Labyrinthine architecture of the tumor parenchymal-stromal border, which was virtually displayed in a plane of one voxel in width. Note that this 3D image was constructed using 30 serial sections to improve the visibility of the tortuous architecture. Bar = 0.5 mm.