Microbial Changes in Subgingival Plaque and Polymicrobial Intracellular Flora in Buccal Cells after Fixed Orthodontic Appliance Therapy: A Preliminary Study

Table 1

Primers used and strains detected by conventional PCR.

Bacteria	Primers	Sequence
Aa 652	OG 155	F: 5′-CATTCTCGGCGAAAAAACTA-3′
	OG 156	R: 5′-CCCATAACCAAGCCACATAC-3′
Aa JP2	OG 155	F: 5′-CATTCTCGGCGAAAAAACTA-3′
	OG 156	R: 5′-CCCATAACCAAGCCACATAC-3′
Aa Y4	OG 155	F: 5′-CATTCTCGGCGAAAAAACTA-3′
	OG 156	R: 5′-CCCATAACCAAGCCACATAC-3′
Pg	OG 94	F: 5′-GAATCAAATACTTCAGCCGTCT-3′
	OG 95	R: 5′-TTGCAGTTCGTATCGGATCT-3′
Pi	OG 53	F: 5′-CGTATCCAACCTTCCCTCC-3′
	OG 54	R: 5′-ATTAGCCGGTCCTTATTCGAAG-3′
Td	OG 348	F: 5′-AGAGAAAGGGTAATTTGAAG-3′
	OG 349	R: 5′-TATTATTGTCCCTTCTTTCTT-3′
Tf	OG 45	F: 5′-GTCGGACTAATACCTCATAAAACA-3′
	OG 46	R: 5′-TCGCCCATTGACCAATATA-3′

AA 652: Aggregatibacter actinomycetemcomitans tp 652.
Aa JP2: Aggregatibacter actinomycetemcomitans tp Jp2.
Aa Y4: Aggregatibacter actinomycetemcomitans tp Y4.
Pg: Porphyromonas gingivalis.
Pi: Prevotella intermedia.
Td: Treponema denticola.
Tf: Tannerella forsythia.