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International Journal of Dentistry
Volume 2015, Article ID 489842, 5 pages
http://dx.doi.org/10.1155/2015/489842
Research Article

In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis

1Oral Medicine Department, Islamic Azad University Dental Branch, Tehran, Iran
2Periodontics Department, Islamic Azad University Dental Branch, Tehran, Iran
3Microbiology Department, Islamic Azad University, Parand Branch, Tehran, Iran
4Islamic Azad University Dental Branch, Tehran, Iran
5Prosthodontics Department, Islamic Azad University Dental Branch, Tehran, Iran

Received 29 January 2015; Revised 2 May 2015; Accepted 4 May 2015

Academic Editor: Timo Sorsa

Copyright © 2015 Arash Azizi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations of Zingiber officinale extract on proliferation of Streptococcus mutans and Streptococcus sanguinis in vitro. Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛ of each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛ of each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as described. Results. The minimum inhibitory concentration (MIC) was 0.02 mg/mL for S. mutans and 0.3 mg/mL for S. sanguinis. The minimum bactericidal concentration (MBC) was 0.04 mg for S. mutans and 0.6 mg for S. sanguinis. Conclusion. Zingiber officinale extract has significant antibacterial activity against S. mutans and S. sanguinis cariogenic microorganisms.