PCR-Based Identification of Oral Streptococcal Species

Table 1

Primer sequences and annealing temperatures.

Primer set	Forward; (5′ to 3′)	Reverse; (5′ to 3′)	Product size (bp)	Annealing temperature (°C)
GDH1	ACAACTGAAACCTTTGCATCTGG	CGGTCGCATCTGTACGGTAA	278	51
GDH2	Same as GDH1	TCAAYTTCCAYGAYGCACCA	391	51
SG1	CCTTGGAGCAAGGAATATTTTGAATCTG	CTTCTTGGCTCGGTTGTGTCAAGCG	695	53
SO3	Same as SG1	AGAGCGATATTGACCACGAATAAAC	732	53
MG1	Same as SG1	CCCGTCTACTTTACCAGAAGTA	715	51
MG2	Same as SG1	GTCTTTTCAGATCATTATCAGTAGGC	706	51
MG3	CGTGGTCGAGTCTATCACAGATCTCGCT	CAGAAACAAAGGTTTCAGTGGTAGAC	788	53
UNIV	GTGSTGCAYGGYTGTCGTCA	ACGTCRTCCMCACCTTCCTC	~200	60

Positive control primers, universal for bacterial 16S rRNA, were chosen based on [17].